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Ca. Brocadia should certainly inhabit freshwater ecosystems, however it also can tolerate a specific standard of salinity. Ca. Scalindua was halophilic anammox bacterium and exists only in saline conditions. Ca. Kuenenia could adjust to an array of salinity and preferred to live in large DIN level problems according to our search. The distribution design of anammox germs could be the consequence of microbial migration and long-lasting adaptation to salinity