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92), venous DBS and venous plasma (r=0.93), and capillary DBS and venous DBS (r=0.97). Ordinary least squares regression was used to characterise (β=0.81) and correct the systematic bias in DBS data (compared to paired plasma). Thereafter, Bland-Altman analysis demonstrated robust agreement between sample-methods. This simple and rapid DBS-based LC-MS/MS assay accurately quantified serum vitamin D metabolites using a paired-sample 'bridging strategy' to correct for the inherent sample-method bias. This simple and rapid DBS-based LC-MS/M