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We succeeded in monitoring the membrane order in the Golgi apparatus, Mitochondria, and plasma membrane in the samein-vivocell and quantitatively comparing them. The phasor-based multi-harmonic unmixing method can help expand the outreach of HSI and democratize its use by the community for it does not require specialized knowledge.To predict the impact of climate change on our beef animals and systems, we need a better understanding of how beef cattle traits are affected by varying weather and frequency of extreme events. We analysed