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05). However, the digestibility of DM, ash, OM, NFE, EE, CF, NDF, ADF, and ADL determined using ADL method were significantly lower than those using the TFC (P less then 0.05). The feces recovery of Cr2O3 and AIA was 95.89% and 97.14%, which were higher than that of ADL (88.90%). In summary, compare with ADL method, TFC, Cr2O3, and AIA methods are more accurate to determine the roughage digestibility of buffalo. Furthermore, Cr2O3 and AIA methods are applicable and convenient to evaluate the roughage digestibility of buffalo under extens