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The opvCRISPR makes it possible for detection at nearly solitary molecule amount in 45 min. We validate this process with 50 SARS-CoV-2 potentially infected clinical examples. The opvCRISPR diagnostic outcomes provide 100% agreement because of the Centers for disorder Control and Prevention (CDC)-approved quantitative RT-PCR assay. The opvCRISPR keeps great potential for SARS-CoV-2 recognition in next-generation point-of-care molecular diagnostics.