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We further studied the in vitro PA imaging capabilities of the Cu-In-Zn-Se/ZnSe/PMAO-g-PEG nanoparticles, which showed a distinct PA signal beyond 1.0 mg ml-1. The current work demonstrated that a moderate amount of Zn doping is necessary for enhancing fluorescence and there is a limit beyond which the fluorescence will be diminished. We also demonstrated the proof of concept that Cu-In-Zn-Se/ZnSe QDs are able to serve as a potential PA imaging contrast agent.Emerging magnetic resonance (MR) guided radiotherapy affords significantly imp