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Customers with autoimmune connective tissue infection (ACTD) may have anti-thyroid peroxidase antibody (TPOAb) and anti-thyroglobulin antibody (TgAb). This study aimed examine the prevalence of thyroid autoantibodies in ACTD customers and controls. Total 10,321 ACTD instances and 12,949 healthy settings were included, and also the prevalence of thyroid autoantibody positivity ended up being higher in ACTD clients than in settings. Subgroup analysis revealed g