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Context Nitriles constitute almost 15% of the molecules observed in the interstellar medium (ISM), surprisingly only two dinitriles have been detected in the ISM so far. The lack of astronomical detections for dinitriles may be partly explained by the absence of laboratory rotational spectroscopic data. Aims Our goal is to investigate the rotational spectrum of glutaronitrile, N≡C-CH2-CH2-CH2-C≡N, in order to allow its possible detection in the ISM. Methods The rotational spectrum of glutaronitrile was measured using two different exper