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This paper describes a near-infrared quantum dot (CuInS2 QD)/antibiotic (vancomycin) nanoparticle-based assay for the Staphylococcus aureus and iron(iii) detection. CuInS2 QDs with good biological tissue permeability and biocompatibility are combined with vancomycin through covalent interaction to form a detection system for two harmful factors. The detection principle of Staphylococcus aureus is mainly the fluorescence quenching caused by the accumulation of CuInS2@Van QDs on the surface of Staphylococcus aureus. The detection princip