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MrM35-4, is required for fungal virulence against insect hosts. MrM35-4 is a secretable chemical and shows a proteolytic activity implicated in assisting fungal penetration of insect cuticles. After gene rescue and overexpression, insect bioassays indicated that MrM35-4 contributes to suppressing insect cuticular and hemocyte melanization activities. Enzymatic cleavage assays revealed that the recombinant prophenoloxidases PPO1 and PPO2 of Drosophila mel
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