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05). An amino acid mutation, T82I, was identified in GyrA and the total mutation rate of the C. difficile strains was 40.8% (71/174), while the mutation rate of the ST81 strains was 95.7% (44/46). Three amino acid mutations including D426N, S366A, and D426V were identified in GyrB and the total mutation rate of GyrB was 39.1%. The double-site mutation in GyrB, S366A+D426V was identified in all the ST81 (n=46) strains. In conclusion, the C. difficile ST81 clone showed high levels of fluoroquinolone resistance in Beijing, China, thus high
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