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This combination was suitable for the normalisation of mRNA expression in placentas supplying GD30, 60 and 90 foetuses of different size. This study has identified reference genes with stable expression in placentae and endometria across multiple gestational days, in tissues associated with foetuses of different size and sex. The results of these experiments highlight the importance of selecting appropriate reference genes for the biological comparison under investigation. This article is protected by copyright. All rights reserved.We