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In this review, the state-of-art strategies of PN hybrids for biosensing are summarized from the view of the role of nanomaterial components, i.e. immobilization matrix, catalyst, and label. Recent advances for the emerging in-field detection applications of PN hybrids with the incorporation of portable hand-held readers and miniaturized devices are then surveyed. The features of PN hybrids for the construction of these miniaturized biosensors are focused. The integration and synergy between proteins and nanomaterials for biosensing is em