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Making use of Matrigel-based three-dimensional tradition practices, we propagated organoids produced by the normal SCJ region, along with metaplastic squamous cells in the TZ. Consisting predominantly of squamous cells, organoids basically exhibited a dense structure. Nevertheless, at the least in a few organoids, a tiny but discrete populace of mucin-producing endocervix cells co-existed adjacent to the squamous cellular population, virtually recapitulating the configuration of SCJ in