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Resolved IAV infection significantly affected subsequently induced AAI on different levels including morphological changes, respiratory leukocytes and lymphocytes as well as the pro-inflammatory cytokine responses, which was clearly diminished. We conclude that IAV has exceptional persisting effects on respiratory immunity with substantial consequences for subsequently induced AAI.Chronic pulmonary aspergillosis (CPA) is a common sequela of pulmonary tuberculosis (T. The diagnosis of CPA is difficult and often misdiagnosed as smear-ne