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t into understanding the role of LT in BA.The marbled flounder (Pseudopleuronectes yokohamae) is a commercial flatfish in East Asia. The aim of this study was to improve its sperm cryopreservation protocol based on the vitality assessment of 7-day and 1-year cryopreserved sperm. Four extenders (extender-1 sucrose solution; extender-2 glucose solution; extender-3 fish Ringer's solution; and extender-4 modified fish Ringer's solution) were tested with a combination of five cryoprotectants (CPAs) (dimethyl sulfoxide Me2SO; glycerol GLY; eth
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