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Substrates had been pretreated ACR through particle size decrease, and SCS with dilution. The maximum methane yield of 0.276 Nm3 CH4 kg-1 VSadded taken place at an ACR of 2 mm as well as 12.5% dilution of SCS, at a ratio of 7525 centered on volatile solids, that was 30.2% and 5.9percent greater compared to SCS and ACR in mono-digestion, respectively. ACR was a substrate of adequate buffer capacity for the AcoD security, even though the SCS, alo