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Rice is an important crop that provides energy and nutrients to humans, which undergoes the aging process, the quality decline is related to the exogenous storage conditions and the change of own enzyme activity. However, due to the complex composition of rice and serious matrix interference, the ageing identification of rice is still challenging. Hence, a novel spinel particles ZnFe2O4@PANI was designed and synthesized for adsorption and determination of vitamin B9, which can be used to distinguish rice in different years and analyze the degree of