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It is well known that the use of shift reagents (SRs) in nuclear magnetic resonance (NMR) studies is substantially limited by an intact blood-brain barrier (BB. The current study aims to develop a method enabling chemical shift imaging in the living rat brain under physiological conditions using an SR, Tm[DOTP] . Hyperosmotic mannitol bolus injection followed by 60min infusion of a Tm[DOTP] containing solution was administered via a catheter inserted into an internal carotid artery. We monitored the homeostasis of physiological parame
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