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transcription and RNA replication, with other roles likely. To better understand how NTRs work, we performed the first comprehensive investigation of the importance of NTR nucleotides in the context of the entire nairovirus replication cycle. We identified both dispensable and critical NTR nucleotides, as well as highlighting the importance of 3' and 5' NTR interactions in virus growth, thus providing the first functional map of the nairovirus NTRs.The HIV-1 envelope glycoprotein (Env) trimer of gp120-gp41 heterodimers mediat