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In this work, bovine serum albumin-based nanoparticles, BSA-ACVA-DOX NPs, were fabricated for ultrasound-driven drug release, and exhibited excellent tissue penetration. Moreover, a pulsatile release of DOX in vivo could be programmed by in vitro (i.e., remotely) switching the ultrasound on and off.Herein, we report a sustainable route for the synthesis of 1,2,3,4-cyclohexanetetracarboxylate from sugar-derived muconic acid and fumaric acid. The key Diels-Alder reaction constructed a cyclohexene framework substituted by four ester group