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3% and 88.6%, respectively, in the [C10mim]NO3 treatment compared to those in the control. The inhibition of amino acid biosynthesis and glyoxylate and dicarboxylate metabolism explained the more serious biotoxicity of [C12mim]NO3 than that of [C10mim]NO3. This study facilitates a better understanding of the environmental safety and ecological risks of ILs.As an important component of dissolved organic matter (DOM), dissolved black carbon (DBC) which is characterized of abundant aromatic and oxygen-containing functional groups, is widely d