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Cholangiocarcinoma (CCA) is difficult to heal due to its inadequate therapy and advanced level phase diagnosis. Thoroughly mechanistic understandings of CCA pathogenesis crucially help improving the treatment success prices. Using three-dimensional (3D) cell culture system provides a few benefits over a conventional two-dimensional (2D) culture since it resembles much more closely to tumefaction. Right here, we aimed to ascertain the 3D CCA spheroids with lowly (KKU-10 and extremely (KKU-213A) metasta
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