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Among them, Fe (Fe2+)-Fe (Fe3O4) forms a metal bond, and Fe (Fe2+)-O (Fe3O4) forms the ionic bond. The results illustrate the interaction between free Fe2+ and Fe3O4 is the reason of the nucleation and agglomeration of Fe3O4 scale and it provides the foundation for the further research on Fe3O4 scale deposition. The efficacy and safety of super-mini percutaneous nephrolithotomy (SMP, 14 Fr) was compared with mini percutaneous nephrolithotomy (MPCNL, 16 Fr) for the treatment of upper urinary tract stones in children ( 14years old). C