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5-MTHF is trusted for the prevention and treatment of different conditions. 5-MTHF is principally created by chemical synthesis. However, the lower production price cannot meet the increasing need. In addition, substance synthesis is potentially damaging to your environment. Despite different microorganisms can synthetize 5-MTHF, a simple yet effective 5-MTHF bioproduction method is lacking due to the tight legislation of 5-MTHF path and restricted metabolic flux toward folic aci