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, transglutaminase (TGase)), and thus achieves building of centimeter-sized tissue with high cell viability and technical stability in vitro within 30 min. Further in vivo implantation regarding the 3D-MAPS-fabricated ear-like structure construct in rabbit designs assisted by flap prefabrication method results in increased structural vascular assistance and strengthened functional success. Therefore, the TGase-enhanced 3D-MAPS shows its prospective and feasibility as a strong biofabrication sys