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To investigate biofouling mitigation techniques, scientific studies have a tendency to give attention to single-species biofilms; selection of organism is a must to creating helpful results. To ascertain a best-practice system for studying antimicrobial remedy for biofilms, with certain desire for biofouling of RO membranes, we answered listed here two questions, each via a unique semi-systematic review 1. Which organisms are generally utilized to try antimicrobial efficacy against b