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In this research, the outcomes of Lactobacillus plantarum-12 exopolysaccharides (L-EPS) and S. flexneri exopolysaccharides (S-EPS) from the S. flexneri CMCC51574 biofilm formation were investigated. The results indicated that L-EPS could reduce the polysaccharides manufacturing into the extracellular polymeric matrix of S. flexneri, and inhibit the biofilm formation of S. flexneri L-EPS could decrease the minimum biofilm removal concentration (MBEC) for