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Here, we provide a luminescence-based strategy to determine ALDOA activity. The assay platform originated by integrating a previously established ALDOA activity assay with a commercial NAD/NADH recognition system, causing an important (12-fold) enhancement in signal/background (S/ compared to previous assay systems. A screening promotion making use of a mixture-based element library exhibited excellent analytical parameters of Z' (0.8) and S/B (~20
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