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© United states Association for Clinical Chemistry 2020. All liberties reserved. For permissions, please email journals.permissions@oup.com.BACKGROUND Rapid recognition of fentanyl during the point-of-care is important. Urine fentanyl concentrations in overdose instances start at single-digit nanograms per milliliter. No fentanyl point-of-care assay with a cutoff at single-digit nanograms per milliliter is available. TECHNIQUES A competitive lateral circulation as