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It's known that the composition of pets' diet programs can affect the structure associated with foods produced, such as milk. Thus, the aim of this study was to assess diets with cactus pear in combination with various forages for F1 Holstein/Zebu cows in the chemical composition and fatty acid profile of milk. Two simultaneous 4 × 4 Latin squares were utilized into the experimental design. Four experimental diet plans were used Diet 1, sorghum silage since the only roughage; Diet 2, so