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Treadmill Incline Benefits Walking at an incline on your treadmill can be a challenging workout and burns more calories than flat treadmill walks. It is essential to monitor your fitness levels and talk to your doctor before you attempt higher incline levels. The muscles that are targeted by incline treadmill running include your glutes as well as your quads and hamstrings. This makes it an excellent treadmill exercise for strengthening and toning these muscles while giving you a great cardio exer