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The proportion of biological parameters was less in the control group as compared to other treatments. This study highlights the potential of supplemental diets to improve the bee's health and colony development when the pollens availability and diversity are insufficient.To demonstrate the large potential of proton minibeam radiotherapy (pMBRT) as a new method to treat tumor diseases, a preclinical proton minibeam radiation facility was designed. It is based on a tandem Van-de-Graaff accelerator providing a 16 MeV proton beam and a 3 G