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Equineembryos exhibit an unusual pattern of glucose tolerance in vitro and are currently cultured in hyperglycaemic conditions. Our main objective was to analyse the effect of different glucose concentrations on in vitro-produced equine embryo development and quality. Experiments comparing in vitro and in vivo produced embryos. Oocytes (n=641) were collected from post-mortem ovaries, matured in vitro and fertilised by intracytoplasmic sperm injection (ICSI). Embryo culture was divided from Day 0 to Day 4 and from Day 4 to Day 9 in three