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The aquatic macroinvertebrate community reflects the ecological status of a river. Typically, some extraction methods have been implemented, but the capture and preservation of organisms are necessary. The techniques of digital image processing applied to ecology have become innovative tools for the characterization of aquatic macroinvertebrates. This research implements a methodology for the processing and classification of four aquatic macroinvertebrates genera Thraulodes, Traverella (Ephemeroptera), Anacroneuria (Plecoptera), and Sm