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To investigate the ultrastructure of the cleavage plane of human cornea after liquid-bubble-prepared tissue for Descemet's membrane endothelial keratoplasty (DMEK). Experimental study with scanning electron microscopy (SEM) and block-face SEM of 18 corneal specimens. Fresh human research donor corneoscleral discs (n=12) were prepared with liquid-bubble technique or examined as untreated controls (n=3). In addition, Descemet's membrane samples, n=3, were obtained in DMEK surgery. The cleavage plane after liquid-bubble Descemet's membra