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We found that the absence, independently or combined, associated with related proteins DEF6 and SWAP-70 strongly enhances differentiation of murine GM-CSF-derived DCs. Contrasting SWAP-70, control through DEF6 will not depend on RHOA activation. DEF6 deficiency causes phrase of this DC-specific transcription element ZBTB46 and extended STAT5 activation in GM-CSF cultures. SWAP-70 and DEF6-mediated restriction of DC differentiation converges mechanistically in the NF-κB path. DEF6 acts at i