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Variability in snake venoms is a well-studied phenomenon. However, sex-based variation of snake venom using siblings is poorly investigated. is responsible for the majority of snakebite accidents in the Brazilian Amazon region. #link# Differences in the venom composition of genus have been linked to several factors such as ontogeny, geographical distribution, prey preferences and sex. Thus, in the current study, venom samples of male and female siblings were analyzed in order to compare their biochemical and biological characteristics. V