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PRACTICAL APPLICATION Experimental investigation of different water status during food processing. Assessment of the potential of multispectral imaging to predict water status. Usage of novel measurement method for food processors. © 2020 Institute of Food Technologists®.The present study aims to study the antibacterial activity of food-grade lipidic nanoemulsion (noncationized/cationized) against Bacillus subtilis (BS). Bactericidal activity was ascertained by studying the morphological transitions on BS using transmission electron micro