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Nanofluidic proteomic technologies had been deployed to assess the phosphorylation of biomarker proteins inside the signaling cascades. Interestingly, copaiba essential oil upregulated the pI3K/Akt/mTOR, MAPK, and JAK/STAT signaling pathways in neuronal cells. The results of copaiba essential oil peaked at 30 min post-treatment, with a half-maximal effective concentration (EC5 of around 80 ng/mL. Treatment with cannabinoid receptor 2 (CB2) agonist AM1241 or the inverse agonist BM