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The production of VFA using as substrate the wastewater produced in a cooked mussel processing factory, containing large COD (13.7 ± 3.2 g COD/L), salt concentrations (21.8 ± 2.8 g NaCl/L) and characterized by low pH (4.6 ± 0.6) was evaluated. This wastewater was fed to a 5-L completely stirred tank reactor operated in continuous mode. The conversion efficiency of its COD content into volatile fatty acids (VFA) was evaluated. The maximum acidification of 43% (total VFA on soluble COD basis) was obtained when an organic loading rate of 2.5