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In this research, rats were intraperitoneally injected with ouabain (0.1-10 µg/kg for 4 times) alone or with subsequent injections of lipopolysaccharide (1 mg/kg). Some rats had been also subjected to disuse for 6 h by hindlimb suspension system. In the diaphragm muscle, persistent ouabain (1 µg/kg) hyperpolarized resting prospective of extrajunctional membrane layer because of specific upsurge in electrogenic transportation activity regarding the 2 Na,K-ATPase isozyme