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Nevertheless, the general contributions of each symbiont to your diet of bark beetles have already been largely unexplored. Therefore, we evaluated the power of three fungal symbionts of hill pine beetle to concentrate nitrogen and produce ergosterol while infecting phloem of a novel number jack pine. Ergosterol had been used as proxy to determine the fungal biomass (hyphal thickness) in today's research. We inoculated 80 trees in 2 forest stands with one of several three fungal species or a