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The concentrations of the analytes showed good linear correlations with the fluorescence quenching, with LODs of 2.60 × 10-6 and 8.14 × 10-7 mol L-1 for glucose and lactate, respectively. The proposed method presented satisfactory intra-day and inter-day repeatabilities, with %RSD values in the range 3.82-6.61%. The enzymatic systems proved to be specific for the analytes and the matrix had no significant influence on the glucose and lactate determinations. The proposed methodology was successfully applied to saliva and serum samples a
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