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The concentration of atrazine in in situ pore water could be reliable to evaluate its bioavailability and herbicidal activity to barnyard grass. C of a herbicide in soil could be an indicator for guiding the practical application rate. © 2022 Society of Chemical Industry. The concentration of atrazine in in situ pore water could be reliable to evaluate its bioavailability and herbicidal activity to barnyard grass. CIPW of a herbicide in soil could be an indicator for guiding the practical application rate. © 2022 Society of Chemical I