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This study aims at uncovering the effects of microRNAs (miRNAs) on the gene and FVIII protein in hemophilia A (HA). -targeting miRNAs were predicted by TargetScan, miRDB, and starBase. MiRNAs, predicted by at least two of the three databases, were selected for further study, and their expressions in the blood of HA patients without mutations and healthy controls were detected. A dual-luciferase reporter assay was performed to verify the binding between hsa-miR-5581-3p/hsa-miR-542-3p and . In addition, the regulation of by hsa-miR-5581-