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Data were analyzed by ANOVA. Dam food intake, pup weight gain, milk flow normalized to dam weight, and milk fat concentration were 17%, 9%, 20%, and 20% greater in the AGL group than in the MA group, respectively (P0.05). Genes involved in lipogenesis and lipid regulation were overexpressed ≤2.76-fold in the mammary gland of AGL dams compared with MA dams (P0.05) and plasma leptin concentration was 39% higher (P=0.008). Milk flow and composition and mammary gene expression of the AL group did not differ from those of the MA group