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Furthermore, RNA interference-mediated knockdown of CYP6CY14, CYP6DC1, and CYP6CZ1 genes significantly increased the sensitivity of Ace-R strain to acetamiprid. Taken together, this study showed that P450 genes especially CYP6CY14, CYP6DC1 and CYP6CZ1 are potentially involved in acetamiprid resistance development in A. gossypii. This study could be useful to understand the molecular basis of acetamiprid resistance mechanism in A. gossypii.Fifteen flavonoids isolated from the Eupatorium adenophorum showed inhibitory activities against acety