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In our research we expressed soluble human NPP4 in Sf9 pest cells and established an enzyme assay making use of diadenosine tetraphosphate (Ap4A) as a substrate. The response item ATP ended up being quantified by luciferin-luciferase response in a 96-well plate structure. The painful and sensitive method exhibited a limit of recognition (LOD) of 14.6 nM, and a Z'-factor of 0.68 suggesting its suitability for high-throughput screening. The new assay had been requested learning enzyme kine
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