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s activated by β1-AR and is under tight control of PDE3 and PDE4. CONCLUSIONS We have established a real-time live cell imaging approach to analyse brown adipocyte cAMP dynamics in real-time using a cAMP biosensor. We showed that during the differentiation from pre-mature to mature murine brown adipocytes, there was a change in PDE-dependent compartmentation of β1-and β3-AR-initiated cAMP responses by PDE3 and PDE4 regulating lipolysis. OBJECTIVES This study aims to evaluate vascular surgeons' knowledge and appreciation of ejaculatory